Bilirubin is formed in the reticuloendothelial system during the degradation of aged erythrocytes. The heme portion from hemoglobin and from other-containing proteins is removed, metabolized to bilirubin, and transported...
Most methods currently used for the quantitative determination of bilirubin are based on the reaction between bilirubin and diazotised sulfanilic acid. In aqueous solution only the direct bilirubin ( conjugated ) bilirubin will react in this manner. On the other hand, in order to estimate total bilirubin the unconjugated bilirubin must be freed from attachment to albumin and rendered water soluble. In Malloy-Evelyn method methanol is used while caffeine/sodium benzoate is used in the Jendrassik-Grof method.
Most methods currently used for the quantitative determination of bilirubin are based on the reaction between bilirubin and diazotised sulfanilic acid. In aqueous solution only the direct bilirubin ( conjugated ) bilirubin will react in this manner. On the other hand, in order to estimate total bilirubin the unconjugated bilirubin must be freed from attachment to albumin and rendered water soluble. In Malloy-Evelyn method methanol is used while caffeine/sodium benzoate is used in the Jendrassik-Grof method.
