Bovine Serum Albumin
CAS # 9048-46-8

Vitro Scient provides a variety of Bovine serum albumins with purities ranging from 95-99%.
Vitro Scient Bovine Serum Albumin (BSA) is a high purity, lyophilized “Fraction V” powder. The albumin is isolated from other plasma protein products and lipids by a unique proprietary, non-solvent procedure.

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PERFORMANCE
High Purity, Low Interference, Low Background. Virtually No Detectable Protease Activity, Ensures Assay Integrity. Superior Solubility/Filterability for Ease of Use.
ref. 1011
5 g
ref. 1012
50 g
ref. 1013
100 g
ref. 1014
250 g
ref. 1015
1 Kg
Please feel free to request your custom package
Vitro Scient provides a variety of Bovine serum albumins with purities ranging from 95-99%.
Vitro Scient Bovine Serum Albumin (BSA) is a high purity, lyophilized “Fraction V” powder. The albumin is isolated from other plasma protein products and lipids by a unique proprietary, non-solvent procedure.
Bovine serum albumin (also known as BSA or “Fraction V”) is a serum albumin protein isolated from cows. Rockland produces BSA in various formats for use in immunology, biochemistry and biotechnology. BSA immunoassay applications include ELISA (Enzyme-Linked Immunosorbent Assay), immunoblots (western blot and dot blot), and immunocytochemistry (immunohistochemistry and immunofluorescence microscopy). Bovine serum albumin (often from a fetal bovine source) is also used as a nutrient in cell and microbial culture. In molecular biology BSA is used to stabilize some restriction enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipet tips, and other vessels.  BSA is considered to be a universal blocking reagent in many applications. This is because BSA does not affect the functions of other proteins (enzymes) that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA in, for instance, the Bradford Protein Assay. BSA is used because of its stability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry.

Highly purified serum albumin protein that has numerous biochemical applications.

  • Bovine Serum Albumin (BSA) is used as as a buffering agent, stabilizer, standard and for blending. Bovine Serum Albumin (BSA) is also a versatile tool against non-specific solid phase interference. As blocking reagent Bovine Serum Albumin (BSA) saturates unoccupied binding sites on the solid phase. Use Bovine Serum Albumin (BSA) typically at a concentration of 0.5 to 3% within the reagent buffer.
  • Protease-sensitive Immunoassays such as RIA, EIA, Fluorescent and Chemiluminescent
  • Protein Standard, Diluent
  • Protein, Conjugate or Enzyme Stabilizer
  • Hybridization
  • Selected Cell Culture applications
SPECIFICATIONS
Molecular weight 68 kD
IgG Contains no detectable IgG.
Appearance Slightly yellow lyophilizate
Protein (from N, according to elementary analysis) ≥97%
Water (K. Fischer) ≤5%
K (flame photometric) ≤0.01%
Fe (AAS) ≤0.001%
Cu (AAS) ≤0.002%
Fatty acids, total (GC) ≤0.2 mg/g
Triglycerides (enzymatically) Not detectable
Immunoglobulines (ELISA) Not detectable
Na (flame photometric) ≤0.5%
Stability 24 months